MLCsplice is a hybrid model based on splicing region-specific, which integrated the best machine learning algorithm from five splicing regions including the exonic region, core donor region, extend donor region, core accept region, and extend accept region.
The genomic coordinate is currently based on GRCh37/GRCh38. The reference/alternative allele should only contain single nucleotide.
MLCsplice provides a score indicated the probability of the splicing effect for all possible splice variants across human protein-coding genes.
Further details can be found in our paper. MLCsplice scores are freely available for all non-commercial applications. If you are planning on using them in a commercial application, please contact us.